Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity.
Identifieur interne : 003587 ( Main/Exploration ); précédent : 003586; suivant : 003588Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity.
Auteurs : Michimi Nakamura [Japon] ; Masato Kuramata ; Isao Kasugai ; Midori Abe ; Shohab YoussefianSource :
- Plant cell reports [ 0721-7714 ] ; 2009.
Descripteurs français
- KwdFr :
- Carbon-oxygen lyases (génétique), Dioxyde de soufre (toxicité), Modèles génétiques (MeSH), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (métabolisme), Sulfure d'hydrogène (toxicité), Technique de Northern (MeSH), Thiols (métabolisme), Thiols (physiologie), Triticum (génétique), Végétaux génétiquement modifiés (effets des médicaments et des substances chimiques), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- effets des médicaments et des substances chimiques : Populus, Végétaux génétiquement modifiés.
- génétique : Carbon-oxygen lyases, Populus, Triticum, Végétaux génétiquement modifiés.
- métabolisme : Populus, Thiols, Végétaux génétiquement modifiés.
- physiologie : Thiols.
- toxicité : Dioxyde de soufre, Sulfure d'hydrogène.
- Modèles génétiques, Technique de Northern.
English descriptors
- KwdEn :
- Blotting, Northern (MeSH), Carbon-Oxygen Lyases (genetics), Hydrogen Sulfide (toxicity), Models, Genetic (MeSH), Plants, Genetically Modified (drug effects), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (drug effects), Populus (genetics), Populus (metabolism), Sulfhydryl Compounds (metabolism), Sulfhydryl Compounds (physiology), Sulfur Dioxide (toxicity), Triticum (genetics).
- MESH :
- chemical , genetics : Carbon-Oxygen Lyases.
- chemical , metabolism : Sulfhydryl Compounds.
- chemical , physiology : Sulfhydryl Compounds.
- chemical , toxicity : Hydrogen Sulfide, Sulfur Dioxide.
- drug effects : Plants, Genetically Modified, Populus.
- genetics : Plants, Genetically Modified, Populus, Triticum.
- metabolism : Plants, Genetically Modified, Populus.
- Blotting, Northern, Models, Genetic.
Abstract
O-acetylserine(thiol) lyase (OASTL), a key enzyme of the plant sulfur assimilatory pathway, catalyses the formation of cysteine from sulfide and O-acetylserine. Transgenic hybrid poplar (Populus sieboldi x P. grandidentata 'Y63') plants expressing cys1, encoding a wheat cytosolic OASTL, were developed in order to examine the role of this enzyme in thiol production following hydrogen sulfide or sulfur dioxide exposure and in the extent of damage induced in the plants by these pollutants. The transgenic cys1 plants accumulated up to several-fold higher cysteine and glutathione levels and were significantly more resistant in terms of foliar damage to the pollutants than WT plants. The transgenic poplar also showed higher tolerance to sulfite and hydrogen peroxide and, interestingly, accumulated several-fold higher sulfite reductase transcripts than WT plants in response to sulfur dioxide. These data clearly demonstrate the important role of OASTL and the sulfur reduction pathway in sulfur and oxidative stress amelioration, and support the notion that transgenic trees resistant to such pollutants can be generated for phytoremediation strategies.
DOI: 10.1007/s00299-008-0635-5
PubMed: 19011861
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity.</title><author><name sortKey="Nakamura, Michimi" sort="Nakamura, Michimi" uniqKey="Nakamura M" first="Michimi" last="Nakamura">Michimi Nakamura</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Chemistry, Faculty of Science, Chiba University, Chiba, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Chemistry, Faculty of Science, Chiba University, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Kuramata, Masato" sort="Kuramata, Masato" uniqKey="Kuramata M" first="Masato" last="Kuramata">Masato Kuramata</name></author><author><name sortKey="Kasugai, Isao" sort="Kasugai, Isao" uniqKey="Kasugai I" first="Isao" last="Kasugai">Isao Kasugai</name></author><author><name sortKey="Abe, Midori" sort="Abe, Midori" uniqKey="Abe M" first="Midori" last="Abe">Midori Abe</name></author><author><name sortKey="Youssefian, Shohab" sort="Youssefian, Shohab" uniqKey="Youssefian S" first="Shohab" last="Youssefian">Shohab Youssefian</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19011861</idno><idno type="pmid">19011861</idno><idno type="doi">10.1007/s00299-008-0635-5</idno><idno type="wicri:Area/Main/Corpus">003736</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003736</idno><idno type="wicri:Area/Main/Curation">003736</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003736</idno><idno type="wicri:Area/Main/Exploration">003736</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity.</title><author><name sortKey="Nakamura, Michimi" sort="Nakamura, Michimi" uniqKey="Nakamura M" first="Michimi" last="Nakamura">Michimi Nakamura</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Chemistry, Faculty of Science, Chiba University, Chiba, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Chemistry, Faculty of Science, Chiba University, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Kuramata, Masato" sort="Kuramata, Masato" uniqKey="Kuramata M" first="Masato" last="Kuramata">Masato Kuramata</name></author><author><name sortKey="Kasugai, Isao" sort="Kasugai, Isao" uniqKey="Kasugai I" first="Isao" last="Kasugai">Isao Kasugai</name></author><author><name sortKey="Abe, Midori" sort="Abe, Midori" uniqKey="Abe M" first="Midori" last="Abe">Midori Abe</name></author><author><name sortKey="Youssefian, Shohab" sort="Youssefian, Shohab" uniqKey="Youssefian S" first="Shohab" last="Youssefian">Shohab Youssefian</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Northern (MeSH)</term><term>Carbon-Oxygen Lyases (genetics)</term><term>Hydrogen Sulfide (toxicity)</term><term>Models, Genetic (MeSH)</term><term>Plants, Genetically Modified (drug effects)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Sulfhydryl Compounds (metabolism)</term><term>Sulfhydryl Compounds (physiology)</term><term>Sulfur Dioxide (toxicity)</term><term>Triticum (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Carbon-oxygen lyases (génétique)</term><term>Dioxyde de soufre (toxicité)</term><term>Modèles génétiques (MeSH)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Sulfure d'hydrogène (toxicité)</term><term>Technique de Northern (MeSH)</term><term>Thiols (métabolisme)</term><term>Thiols (physiologie)</term><term>Triticum (génétique)</term><term>Végétaux génétiquement modifiés (effets des médicaments et des substances chimiques)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carbon-Oxygen Lyases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Sulfhydryl Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Sulfhydryl Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Hydrogen Sulfide</term><term>Sulfur Dioxide</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carbon-oxygen lyases</term><term>Populus</term><term>Triticum</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term><term>Thiols</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Thiols</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Dioxyde de soufre</term><term>Sulfure d'hydrogène</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Models, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Modèles génétiques</term><term>Technique de Northern</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">O-acetylserine(thiol) lyase (OASTL), a key enzyme of the plant sulfur assimilatory pathway, catalyses the formation of cysteine from sulfide and O-acetylserine. Transgenic hybrid poplar (Populus sieboldi x P. grandidentata 'Y63') plants expressing cys1, encoding a wheat cytosolic OASTL, were developed in order to examine the role of this enzyme in thiol production following hydrogen sulfide or sulfur dioxide exposure and in the extent of damage induced in the plants by these pollutants. The transgenic cys1 plants accumulated up to several-fold higher cysteine and glutathione levels and were significantly more resistant in terms of foliar damage to the pollutants than WT plants. The transgenic poplar also showed higher tolerance to sulfite and hydrogen peroxide and, interestingly, accumulated several-fold higher sulfite reductase transcripts than WT plants in response to sulfur dioxide. These data clearly demonstrate the important role of OASTL and the sulfur reduction pathway in sulfur and oxidative stress amelioration, and support the notion that transgenic trees resistant to such pollutants can be generated for phytoremediation strategies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19011861</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>28</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity.</ArticleTitle><Pagination><MedlinePgn>313-23</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-008-0635-5</ELocationID><Abstract><AbstractText>O-acetylserine(thiol) lyase (OASTL), a key enzyme of the plant sulfur assimilatory pathway, catalyses the formation of cysteine from sulfide and O-acetylserine. Transgenic hybrid poplar (Populus sieboldi x P. grandidentata 'Y63') plants expressing cys1, encoding a wheat cytosolic OASTL, were developed in order to examine the role of this enzyme in thiol production following hydrogen sulfide or sulfur dioxide exposure and in the extent of damage induced in the plants by these pollutants. The transgenic cys1 plants accumulated up to several-fold higher cysteine and glutathione levels and were significantly more resistant in terms of foliar damage to the pollutants than WT plants. The transgenic poplar also showed higher tolerance to sulfite and hydrogen peroxide and, interestingly, accumulated several-fold higher sulfite reductase transcripts than WT plants in response to sulfur dioxide. These data clearly demonstrate the important role of OASTL and the sulfur reduction pathway in sulfur and oxidative stress amelioration, and support the notion that transgenic trees resistant to such pollutants can be generated for phytoremediation strategies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nakamura</LastName><ForeName>Michimi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Molecular Chemistry, Faculty of Science, Chiba University, Chiba, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kuramata</LastName><ForeName>Masato</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kasugai</LastName><ForeName>Isao</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Abe</LastName><ForeName>Midori</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Youssefian</LastName><ForeName>Shohab</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>11</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013438">Sulfhydryl Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0UZA3422Q4</RegistryNumber><NameOfSubstance UI="D013458">Sulfur Dioxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.47</RegistryNumber><NameOfSubstance UI="C018725">O-acetylhomoserine (thiol)-lyase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.2.-</RegistryNumber><NameOfSubstance UI="D019757">Carbon-Oxygen Lyases</NameOfSubstance></Chemical><Chemical><RegistryNumber>YY9FVM7NSN</RegistryNumber><NameOfSubstance UI="D006862">Hydrogen Sulfide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019757" MajorTopicYN="N">Carbon-Oxygen Lyases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006862" MajorTopicYN="N">Hydrogen Sulfide</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008957" MajorTopicYN="N">Models, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013438" MajorTopicYN="N">Sulfhydryl Compounds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013458" MajorTopicYN="N">Sulfur Dioxide</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>06</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>10</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>10</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>11</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>4</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>11</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19011861</ArticleId><ArticleId IdType="doi">10.1007/s00299-008-0635-5</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Exp Bot. 2004 Apr;55(398):837-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15020643</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1949 Jan;24(1):1-15</Citation><ArticleIdList><ArticleId IdType="pubmed">16654194</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Sep;136(1):2443-50</Citation><ArticleIdList><ArticleId IdType="pubmed">15375200</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2008 Jun;147(2):562-72</Citation><ArticleIdList><ArticleId IdType="pubmed">18441224</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2002 Sep;31(6):729-40</Citation><ArticleIdList><ArticleId IdType="pubmed">12220264</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Environ. 2007 Apr;30(4):447-55</Citation><ArticleIdList><ArticleId IdType="pubmed">17324231</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1998 Oct;118(2):471-82</Citation><ArticleIdList><ArticleId IdType="pubmed">9765532</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Jul;126(3):973-80</Citation><ArticleIdList><ArticleId IdType="pubmed">11457948</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2007 May;50(4):696-709</Citation><ArticleIdList><ArticleId IdType="pubmed">17425719</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1993 Nov;4(5):759-69</Citation><ArticleIdList><ArticleId IdType="pubmed">8275097</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Jul;126(3):1001-11</Citation><ArticleIdList><ArticleId IdType="pubmed">11457951</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Environ. 2006 Mar;29(3):382-95</Citation><ArticleIdList><ArticleId IdType="pubmed">17080593</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation><ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1994 Feb;104(2):597-604</Citation><ArticleIdList><ArticleId IdType="pubmed">12232109</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1997;203(3):362-72</Citation><ArticleIdList><ArticleId IdType="pubmed">9431683</ArticleId></ArticleIdList></Reference><Reference><Citation>Crit Rev Toxicol. 1984;13(1):25-97</Citation><ArticleIdList><ArticleId IdType="pubmed">6378532</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1999 Jul;11(7):1277-92</Citation><ArticleIdList><ArticleId IdType="pubmed">10402429</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1999 Feb;39(3):527-37</Citation><ArticleIdList><ArticleId IdType="pubmed">10092180</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Abe, Midori" sort="Abe, Midori" uniqKey="Abe M" first="Midori" last="Abe">Midori Abe</name><name sortKey="Kasugai, Isao" sort="Kasugai, Isao" uniqKey="Kasugai I" first="Isao" last="Kasugai">Isao Kasugai</name><name sortKey="Kuramata, Masato" sort="Kuramata, Masato" uniqKey="Kuramata M" first="Masato" last="Kuramata">Masato Kuramata</name><name sortKey="Youssefian, Shohab" sort="Youssefian, Shohab" uniqKey="Youssefian S" first="Shohab" last="Youssefian">Shohab Youssefian</name></noCountry><country name="Japon"><noRegion><name sortKey="Nakamura, Michimi" sort="Nakamura, Michimi" uniqKey="Nakamura M" first="Michimi" last="Nakamura">Michimi Nakamura</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003587 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003587 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19011861
|texte= Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19011861" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |